Does Yersinia pestis exhibit bipolar staining
Y pestis is a non-motile Gram-negative rod that grows as a facultative anaerobe on a variety of bacteriologic media. It exhibits striking bipolar staining with special stains like Wright, Giemsa, Wayson, or methylene blue (Figure 19-1).
Where is Burkholderia pseudomallei found
In soil in Southeast Asia and Northern Australia, Burkholderia pseudomallei, a highly pathogenic bacterium that causes melioidosis, is frequently present 1 and 2.
What does it mean by bipolar staining
Pasteurella multocida is a small, nonmotile Gram-negative coccobacillus that frequently displays bipolar staining, in which the ends of the bacilli stain more intensely than the middle.
Does Yersinia enterocolitica grow on MacConkey Agar
Y. enterocolitica and pseudotuberculosis grow on MacConkey agar, are bile tolerant, ferment glucose but not lactose, are oxidase negative, and convert nitrate to nitrite. 23 September 2021
What is Wayson stain test
The safety pin appearance is identified using the Wayson and Wright-Giemsa stains, which offer contrast staining to make bacteria, tissue, and blood cell components visible.
Is Yersinia pestis Gram positive or negative
It appears as plump, gram-negative coccobacilli that are mostly seen as single cells or pairs, and may exhibit bipolar staining from a direct specimen if stained with Wrights or Giemsa stains.15 Aug 2005 Yersinia pestis is a nonmotile, slow-growing, facultative organism classified in the family Enterobacteriaceae.
What is melioidosis
Burkholderia pseudomallei, which causes the uncommon bacterial infection melioidosis, also known as Whitmores disease, is contracted through direct contact with contaminated soil and surface waters.
What is the principle behind the Gram stain reaction
Gram-positive microorganisms have higher peptidoglycan content, whereas gram-negative organisms have higher lipid content. The basic principle of gram staining involves the ability of the bacterial cell wall to retain the crystal violet dye during solvent treatment.
What bacteria has bipolar staining
Although most Enterobacteriaceae can exhibit a bipolar staining appearance when found in clinical specimens, bipolar staining is typically described as a Yersinia pestis characteristic.
What is bipolar staining Yersinia
In the family Enterobacteriaceae, Yersinia pestis is a nonmotile, slow-growing, facultative organism that primarily appears as single or pairs of plump, gram-negative coccobacilli. If stained with Wrights or Giemsa stains from a direct specimen, it may show bipolar staining.15 Aug 2005
Which organism has safety pin appearance
Burkholderia pseudomallei, a gram-negative bacillus with a distinctive “safety pin” appearance (bipolar staining) on Gram staining, is the causative agent of melioidosis.
What kills Burkholderia pseudomallei
The US Centers for Disease Control and Prevention (CDC) advises meropenem or ceftazidime as intravenous treatments and TMP-SMX or doxycycline as oral treatments for pseudomallei. Other options include aminoglycosides, tetracycline, chloramphenicol, and amoxicillin-clavulanate.
How is melioidosis transmitted
The infection is thought to spread between people and animals through contact with contaminated soil, particularly through skin abrasions, ingestion of contaminated food or water, ingestion of contaminated soil, or inhalation of contaminated dust or water droplets.
Can melioidosis be cured
The type of infection and the course of treatment will affect the long-term outcome. When melioidosis infection is diagnosed, the disease can be treated with the use of appropriate medication.
Which is the most commonly reported organism in cases of laboratory acquired bacterial infection
Hepatitis B virus (HBV) is the most frequent cause of laboratory-acquired infection among the common blood-associated viruses [1].
Where does B cepacia come from
In patients who are vulnerable to illness, Burkholderia cepacia, also known as B. cepacia, is a group of bacteria that can cause serious respiratory infections.
What are the symptoms of melioidosis
Localized infections can cause pain, localized swelling, skin infection, ulceration, and abscess formation (with accompanying fever and muscle aches and pains). Disseminated melioidosis infection symptoms can include fever, weight loss, stomach or chest pain, muscle or joint pain, headache, and seizures.
On which selective media will Burkholderia pseudomallei grow as pink wrinkled colonies
Burkholderia pseudomallei, the bacterium that causes melioidosis, can be isolated and characterized using Ashdowns medium, a selective culture medium.